BMP2 Protein (AA 283-396)

Catalog No. ABIN7677192

This Recombinant BMP2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for BMP2 Protein (AA 283-396) (ABIN7677192)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Purity: > 90 %

Product Details

Protein Characteristics: AA 283-396

Sequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Purification: > 90% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is ≤ 100 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Sodium acetate ( pH 3.5)

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: BMP-2

Background: BMP-2, also known as Bone morphogenetic protein 2, is multi-functional growth factor that belongs to the transforming growth factor beta (TGF beta) superfamily. It plays an important role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility. Recombinant human BMP2 was expressed in E. coli and purified by conventional chromatography techniques.

Molecular Weight: 13 kDa (115aa)

NCBI Accession: NP_001191

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation